Analysis Note
ControlTestis, connective tissue
Application
Research CategoryCell Structure
Immunohistochemistry on acetone fixed paraffin-embedded tissues. Formalin fixation is not recommended. Antigen recovery is treatment with 0.04% trypsin in 0.01M CaCl(2) 0.05M Tris-HCL pH 7.6, 37°C for 10 minutes before the non-specific peroxidase block.
EIA
Optimal working dilutions must be determined by end user.
Research Sub CategoryECM Proteins
Anti-Collagen Type VI Antibody, clone VI-26 detects level of Collagen Type VI & has been published & validated for use in ELISA, IH(P).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Protein A purified
Format: Purified
Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.
Specificity
Specifically reacts with the hCL(VI). This is a purified mouse monoclonal antibody to human type VI collagen. VI-26 does not react with denatured or reduced type VI collagen, although this antibody does recognize the the triple helix consisting of alpha1(VI), alpha 2(VI) alpha3(gamma). The epitope has not been mapped and the monoclonal is know not to react with collagens I, II, III, IV or V in native assays.
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product has met the following criteria to qualify for the following awards: